Marmoset model of trauma-induced epileptogenesis

Olga Bukhtiyarova, Igor Timofeev
Clinical Neurophysiology, Volume 130, Issue 8, August 2019, Page e117

We investigated brain electrographic activity in freely behaving marmosets before and after cortical penetrating wound. Based on visual examination of the multi-channel cortical and hippocampal LFP recordings and muscle activity, we were able to distinguish six behavioural states: active and quiet wake, an intermediate state, NREM2, SWS and REM sleep. We developed a procedure for automatic classification of states of vigilance in marmosets based on different LFP power bands and muscle tone with the use of self-organizing maps. Marmosets were typically awake during the day with few occasional naps and had 12–18 sleep cycles during night. In order to evaluate them as a model of human trauma-induced epileptogenesis, we produced cortical penetrating wound in 3 marmosets. All of them developed electrographic epileptiform activity within the first weeks that was primarily local. Its secondary generalization led to behavioural seizures that started to manifest 2–4 weeks after the onset of electrographic events.